Gene Probes,9780896038851

Gene Probes

by ;
ISBN13: 9780896038851
ISBN10: 0896038858
Format: Hardcover
Pub. Date: 2001-09-01
Publisher(s): Humana Pr Inc
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Summary

Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.

Table of Contents

Preface v
Contributors ix
Target Format and Hybridization Conditions
1(12)
Alex Reid
End Labeling Procedures: An Overview
13(6)
Elena Hilario
Photobiotin Labeling
19(4)
Elena Hilario
Nick Translation
23(4)
Alex Reid
Random Primed Labeling
27(2)
Alex Reid
Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization
29(14)
Philip Hugenholtz
Gene W. Tyson
Linda L. Blackall
Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors
43(18)
Irene Orlow
Carlos Cordon-Cardo
The β-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites
61(24)
Alexis Mendoza-Leon
Luis Luis
Clara Martinez
Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma
85(8)
Ross D. Brown
P. Joy Ho
Detection of Chlamydia trachomatis by DNA Amplification
93(8)
Peter Timms
Sarah Mathews
Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes
101(12)
Alex van Belkum
Nicole van den Braak
Leen-Jan van Doorn
Hubert Endtz
Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction
113(24)
Gerard J. Nuovo
Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes
137(12)
Teresa Bisucci
Tim D. Hewitson
Ian A. Darby
Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4
149(10)
Stefan Radajewski
J. Colin Murrell
Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes)
159(12)
Gillian D. Lewis
Sally A. Anderson
Susan J. Turner
Analysis of 16S--23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species
171(8)
Jongsik Chun
Irma N. G. Rivera
Rita R. Colwell
Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes
179(8)
Laura G. Leff
In Situ Hybridization of Burkholderia cepacia Cells
187(6)
Christopher J. McNamara
Laura G. Leff
Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil
193(18)
John Sanseverino
Alice C. Layton
Gary S. Sayler
Detection of Single-Copy Genes in DNA from Transgenic Plants
211(12)
Lee C. Garratt
Matthew S. McCabe
J. Brian Power
Michael R. Davey
Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission
223(12)
Claudio Bonghi
Benedetto Ruperti
Pietro Tonutti
Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food
235(10)
Keith A. Lampel
Palmer A. Orlandi
Polymerase Chain Reaction for Detection of Listeria monocytogenes
245(14)
Debra K. Winters
Index 259

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