Environmental Microbiology

by ; ; ;
Format: Hardcover
Pub. Date: 2000-02-23
Publisher(s): Elsevier Science
  • Free Shipping Icon

    This Item Qualifies for Free Shipping!*

    *Excludes marketplace orders.

List Price: $136.50

Rent Textbook

Select for Price
There was a problem. Please try again later.

New Textbook

We're Sorry
Sold Out

Used Textbook

We're Sorry
Sold Out

eTextbook

We're Sorry
Not Available

How Marketplace Works:

  • This item is offered by an independent seller and not shipped from our warehouse
  • Item details like edition and cover design may differ from our description; see seller's comments before ordering.
  • Sellers much confirm and ship within two business days; otherwise, the order will be cancelled and refunded.
  • Marketplace purchases cannot be returned to eCampus.com. Contact the seller directly for inquiries; if no response within two days, contact customer service.
  • Additional shipping costs apply to Marketplace purchases. Review shipping costs at checkout.

Summary

The field of environmental microbiology encompasses aspects of several areas of study including microbial ecology, molecular genetics, and environmental science. Environmental Microbiology is the first book to offer a comprehensive discussion of this field as a discipline, which the authors define as the study of microbial fate and activity in air, water, and soil, and the resulting impact on human health and welfare. While the roots of environmental microbiology can be traced to sanitary engineering (water and wastewater treatment), the field has grown to include other practical issues such as bioremediation, the control of known and emerging waterborne pathogens, microbial risk assessment, and environmental biotechnology. Five general areas are emphasized in this text: (i) Foundation chapters, (ii) microbial environments, (iii) detection of microbial activity, (iv) the impact of microbial activity on the environment in terms of nutrient cycling and pollutant fate, and (v) detection and control of pathogens in the environment. Designed for courses at senior undergraduate and graduate levels, Environmental Microbiology will also serve as an essential reference for environmental microbiologists, microbial ecologists, and environmental engineers, as well as those interested in epidemiology, water and wastewater treatment, and biotechnology.

Table of Contents

Preface xv
The Authors xvii
Contributing Authors xix
Introduction to Environmental Microbiology
Raina M. Maier
Ian L. Pepper
Charles P. Gerba
Introduction
1(1)
An Historical Perspective
1(3)
Modern Environmental Microbiology
4(1)
Purpose and Organization of This Text
5(2)
Microorganisms in the Environment
Kelly A. Reynolds
Ian L. Pepper
Viruses
7(12)
Historical Outbreaks and Discovery
8(1)
Indigenous Virus Populations in the Environment
9(1)
Distinguishing Characteristics of Viruses and Their Evolution
10(2)
Structure and Classification
12(2)
Infective Nature of Viruses
14(2)
The Metabolic State of Viruses
16(1)
Methods of Virus Infection and Replication
16(3)
Bacteria
19(12)
The Bacterial Lifestyle
19(1)
Size and Shape of Bacteria
19(1)
Bacterial Form
20(1)
Structure of the Bacterial Cell
21(4)
Plasmid-Chromosome Relationships
25(2)
Bacterial Metabolism
27(4)
Fungi
31(3)
The Fungal Lifestyle
31(1)
Taxonomic Diversity of Fungi
31(1)
Size and Shape of Fungi
32(1)
Structure of the Fungal Cell
32(2)
Algae
34(1)
Structure of Algae
34(1)
Protozoa
34(9)
Protozoan Structure
35(1)
Feeding Mechanisms
36(1)
Cycles of Reproduction
36(1)
Ecology
37(2)
Disease-Causing Protozoa
39(1)
Questions and Problems
40(1)
References and Recommended Readings
40(3)
Bacterial Growth
Raina M. Maier
Growth in Pure Culture in a Flask
43(8)
The Lag Phase
44(1)
The Exponential Phase
44(4)
The Stationary Phase
48(1)
The Death Phase
48(1)
Effect of Substrate Concentration on Growth
49(2)
Continuous Culture
51(1)
Growth under Aerobic Conditions
52(3)
Growth under Anaerobic Conditions
55(1)
Growth in the Environment
56(5)
The Lag Phase
56(2)
The Exponential Phase
58(1)
The Stationary and Death Phases
58(1)
Questions and Problems
59(1)
References and Recommended Readings
59(2)
Terrestrial Environments
Raina M. Maier
Ian L. Pepper
Introduction
61(1)
Porous Media
61(9)
The Solid Phase
62(4)
Organic Matter
66(2)
The Liquid Phase
68(2)
Soil Atmosphere
70(1)
Soil and Subsurface Environments
70(4)
Surface Soils
71(2)
Vadose Zone
73(1)
Saturated Zone
74(1)
General Characteristics of Microorganisms in Porous Media
74(3)
Distribution of Microorganisms in Porous Media
75(1)
Metabolic States of Bacteria in Porous Media
76(1)
Microbial Activities in Porous Media
77(2)
Role of Microorganisms in Surface Soil Formation
77(1)
Role of Microorganisms in Nutrient Cycling
78(1)
Role of Microorganisms in Pollution Abatement via Bioremediation
78(1)
Role of Microorganisms in Municipal Waste Disposal
79(1)
Microorganisms in Surface Soils
79(4)
Bacteria
79(1)
Fungi
80(1)
Algae
81(1)
Protozoa
82(1)
Microorganisms in Shallow Subsurface Environments
83(2)
Microorganisms in Deep Subsurface Environments
85(6)
Microorganisms in the Deep Vadose Zone
86(1)
Microorganisms in the Deep Saturated Zone
86(2)
Questions and Problems
88(1)
References and Recommended Readings
89(2)
Aeromicrobiology
Scot E. Dowd
Raina M. Maier
Introduction
91(1)
Important Airborne Pathogens
91(2)
Important Airborne Toxins
93(1)
Nature of Bioaerosols
93(1)
The Atmosphere
94(1)
Aeromicrobiological Pathway
95(5)
Launching
95(1)
Transport
96(2)
Deposition
98(2)
Mathematical Modeling
100(3)
Point Source Modeling
101(1)
Area Source Modeling
102(1)
Indoor Air Modeling
102(1)
Sampling Devices for the Collection of Bioaerosols
103(2)
Impingement
103(1)
Impaction
104(1)
Centrifugation
104(1)
Filtration and Deposition
105(1)
Microbial Survival in the Air
105(2)
Relative Humidity
106(1)
Temperature
106(1)
Radiation
106(1)
Oxygen, OAF, and Ions
107(1)
Extramural Aeromicrobiology
107(4)
Agriculture
107(2)
Waste Disposal
109(1)
Germ Warfare
109(2)
Intramural Aeromicrobiology
111(2)
Buildings
111(1)
Spaceflight
112(1)
Public Health
113(1)
Hospitals and Laboratories
113(1)
Bioaerosol Control
113(2)
Ventilation
113(1)
Filtration
114(1)
Biocidal Control
114(1)
Isolation
114(1)
Biosafety in the Laboratory
115(5)
Biological Safety Cabinets
115(3)
Biosafety Laboratories
118(1)
Biological Agent Classification
119(1)
Other Areas of Interest
120(3)
Questions and Problems
120(1)
References and Recommended Readings
121(2)
Aquatic and Extreme Environments
Scot E. Dowd
David C. Herman
Raina M. Maier
Introduction
123(1)
Microbial Habitats in the Aquatic Environment
123(7)
Planktonic Environment
123(2)
Benthic Habitat
125(1)
Microbial Mats
126(2)
Biofilms
128(2)
Aquatic Environments
130(10)
Freshwater Environments
130(6)
Brackish Water
136(1)
Marine Water
137(3)
Subterranean Water
140(1)
Environmental Determinants That Gover Extreme Environments
140(3)
Air-Water Interface
140(1)
High Temperature
141(1)
High Solute
141(1)
Low pH
142(1)
High Pressure
142(1)
No Nutrient
142(1)
Aquatic Microbes in the News
143(4)
Giant Marine Bacteria
143(1)
Aquatic Microbes: Food for the Future
143(2)
Questions and Problems
145(1)
References and Recommended Readings
145(2)
Microbial Transport
Deborah T. Newby
Ian L. Pepper
Raina M. Maier
Introduction
147(1)
Factors Affecting Microbial Transport
148(14)
Microbial Filtration
148(1)
Physiological State
149(2)
Microbial Adhesion---The Influence of Cell Surface Properties
151(4)
Impact of pH on Microbial Transport
155(1)
Impact of Ionic Strength on Transport
156(1)
Cellular Appendages
157(1)
Sedimentation
158(1)
Hydrogeological Factors
158(3)
Persistence and Activity of Introduced Microbes
161(1)
Factors Affecting Transport of DNA
162(1)
Novel Approaches to Facilitate Microbial Transport
163(3)
Ultramicrobacteria
163(1)
Surfactants
163(1)
Gene Transfer
164(2)
Other Ideas
166(1)
Methodology for Studying Transport
166(6)
Columns
166(2)
Lysimeters
168(1)
Field Studies
169(2)
Tracers
171(1)
Models for Microbial Transport
172(5)
Advection-Dispersion Models
173(1)
Filtration Models
173(1)
Questions and Problems
173(1)
References and Recommended Readings
174(3)
Environmental Sample Collection and Processing
Ian L. Pepper
Charles P. Gerba
Raina M. Maier
Soils and Sediments
177(9)
Sampling Strategies and Methods for Surface Soils
177(2)
Sampling Strategies and Methods for the Subsurface
179(2)
Sample Processing and Storage
181(5)
Water
186(4)
Sampling Strategies and Methods for Water
186(1)
Processing of Water Samples for Virus Analysis
186(2)
Processing Water Samples for Detection of Bacteria
188(1)
Processing Water Samples for Detection of Protozoan Parasites
189(1)
Detection of Microorganisms on Fomites
190(5)
Questions and Problems
194(1)
References and Recommended Readings
194(1)
Microscopic Techniques
Timberley M. Roane
Ian L. Pepper
History of Microscopy
195(1)
Theory of Microscopy
195(2)
Basic Microscopic Techniques
197(3)
Types of Microscopes
197(2)
Preparation of Samples for Microscopic Observation
199(1)
In Situ Soil Microscopy
200(1)
In Situ Detection with Molecular Probes and Epifluoresence Microscopy
201(1)
Direct Bacterial Counts
202(2)
Estimation of Biomass Using Microscopic Counts
204(1)
Advanced Microscopic Techniques
204(9)
Polarization Microscopy
204(1)
Electron Microscopy
205(4)
Confocal Scanning Microscopy
209(1)
Photography
209(1)
Other Imaging Systems
210(1)
Questions and Problems
211(1)
References and Recommended Readings
211(2)
Cultural Methods
Karen C. Josephson
Charles P. Gerba
Ian L. Pepper
Cultural Methods for Enumeration of Bacteria
213(5)
Enumeration and Isolation Techniques
213(1)
Plating Methods
214(3)
Most Probable Number Technique
217(1)
Cultural Media for Bacteria
218(6)
General Media Used for Culturing Bacteria
218(6)
Cultural Methods for Fungi
224(1)
Cultural Methods for Algae and Cyanobacteria
225(2)
Cell Culture-Based Detection Methods for Viruses
227(8)
Questions and Problems
232(1)
References and Recommended Readings
232(3)
Physiological Methods
David C. Herman
Raina M. Maier
Introduction
235(1)
Measuring Microbial Activity in Pure Culture
236(7)
Substrate Disappearance
236(3)
Terminal Electron Acceptors
239(3)
Cell Mass
242(1)
Carbon Dioxide Evolution
242(1)
Choosing the Appropriate Activity Measurement for Environmental Samples
243(1)
Carbon Respiration
243(14)
Measurement of Respiration Gases, CO2 and O2, in Laboratory and Field Studies
245(3)
The Application of Respiration Measurements in Environmental Microbiology
248(6)
Tracer Studies to Determine Heterotrophic Potential
254(2)
Anaerobic Respiration as an Indicator of Microbial Activity
256(1)
Incorporation of Radiolabeled Tracers into Cellular Macromolecules
257(1)
Incorporation of Thymidine into DNA
257(1)
Incorporation of Leucine into Protein
258(1)
Adenylate Energy Charge
258(1)
Enzyme Assays
259(8)
Dehydrogenase Assay
261(2)
Esterase Assay
263(1)
Questions and Problems
263(1)
References and Recommended Readings
263(4)
Immunological Methods
Scot E. Dowd
Raina M. Maier
Introduction
267(1)
What Is an Antibody?
267(6)
Antibody Diversity
268(1)
Antibody Specificity
269(1)
Antibody Affinity
269(1)
Polyclonal and Monoclonal Antibodies
270(2)
Antiglobulins
272(1)
Immunoassays
273(14)
Fluorescent Immunolabeling
274(2)
Enzyme-Linked Immunosorbent Assays
276(1)
Competitive ELISA
277(1)
Immunomagnetic Separation Assays
278(1)
Western Immunoblotting Assays
279(1)
Immunoaffinity Chromatography Assays
280(2)
Immunocytochemical Assays
282(1)
Immunoprecipitation Assays
282(2)
Questions and Problems
284(1)
References and Recommended Readings
284(3)
Nucleic Acid-Based Methods of Analysis
Elizabeth M. Marlowe
Karen L. Josephson
Ian L. Pepper
Structure and Complementarity of Nucleic Acids
287(2)
Obtaining Microbial Nucleic Acids from the Environment
289(1)
Extracting Community Bacterial DNA from Soil Samples
289(1)
Nucleic Acid-Based Methods
290(29)
Gene Probes and Probing
290(4)
Polymerase Chain Reaction
294(10)
Recombinant DNA Techniques
304(3)
Restriction Fragment Length Polymorphism Analysis
307(3)
Denaturing/Temperature Gradient Gel Electrophoresis
310(1)
Plasmid Analysis
311(1)
Reporter Genes
312(3)
Questions and Problems
315(1)
References and Recommended Readings
316(3)
Biogeochemical Cycling
Raina M. Maier
Introduction
319(2)
Biogeochemical Cycles
319(1)
Gaia Hypothesis
319(2)
Carbon Cycle
321(10)
Carbon Reservoirs
321(1)
Carbon Fixation and Energy Flow
322(1)
Carbon Respiration
322(9)
Nitrogen Cycle
331(9)
Nitrogen Reservoirs
332(1)
Nitrogen Fixation
332(1)
Ammonium Assimilation (Immobilization) and Ammonification (Mineralization)
333(2)
Nitrification
335(1)
Nitrate Reduction
336(4)
Sulfur Cycle
340(7)
Sulfur Reservoirs
340(1)
Assimilatory Sulfate Reduction and Sulfur Mineralization
341(1)
Sulfur Oxidation
342(2)
Sulfur Reduction
344(1)
Questions and Problems
345(1)
References and Recommended Readings
345(2)
Consequences of Biogeochemical Cycles Gone Wild
David C. Herman
Raina M. Maier
Introduction
347(1)
Microbially Influenced Corrosion
347(3)
Metal Corrosion
347(2)
Concrete Corrosion
349(1)
Acid Mine Drainage and Metal Recovery
350(2)
Acid Mine Drainage
350(1)
Metal Recovery and Desulfurization of Coal
351(1)
Biomethylation of Metals and Metalloids
352(2)
Nitrous Oxide and Earth's Atmosphere
354(2)
Nitrate Contamination of Groundwater
356(2)
Biogenesis of Halomethanes and Other Organohalogens
358(1)
Composting
358(5)
Questions and Problems
360(1)
References and Recommended Readings
360(3)
Microorganisms and Organic Pollutants
Raina M. Maier
Introduction
363(1)
Environmental Law
364(1)
The Overall Process of Biodegradation
365(3)
Relationship between Contaminant Structure, Toxicity, and Biodegradability
368(7)
Genetic Potential
370(1)
Bioavailability
370(2)
Contaminant Structure
372(1)
Toxicity
373(2)
Environmental Factors Affecting Biodegradation
375(2)
Oxygen
375(1)
Organic Matter Content
375(1)
Nitrogen
376(1)
Other Environmental Factors
376(1)
Biodegradation of Organic Pollutants
377(17)
Pollutant Sources and Types
377(1)
Aerobic Conditions
377(12)
Anaerobic Conditions
389(5)
Bioremediation
394(9)
Addition of Oxygen or Other Gases
396(1)
Nutrient Addition
396(3)
Stimulation of Anaerobic Degradation Using Alternative Electron Acceptors
399(1)
Addition of Surfactants
399(1)
Addition of Microorganisms or DNA
399(1)
Questions and Problems
400(1)
References and Recommended Readings
400(3)
Microorganisms and Metal Pollutants
Timberley M. Roane
Ian L. Pepper
Cause for Concern
403(1)
Metals Defined
404(1)
Sources of Metals
405(1)
Metal Bioavailability in the Environment
406(2)
Metal Chemistry
407(1)
Cation Exchange Capacity
407(1)
Redox Potential
408(1)
pH
408(1)
Metal Toxicity Effects on the Microbial Cell
408(1)
Mechanisms of Microbial Metal Resistance and Detoxification
408(5)
General Mechanisms of Metal Resistance
410(1)
Metal-Dependent Mechanisms of Resistance
411(2)
Methods for Studying Metal-Microbial Interactions
413(2)
Adverse Effects of Microbial Metal Transformations
415(1)
The Benefits of Metal-Microbial Interactions
416(2)
Physical/Chemical Methods of Metal Remediation
418(1)
Innovative Microbial Approaches in the Remediation of Metal-Contaminated Soils and Sediments
418(3)
Innovative Microbial Approaches in the Remediation of Metal-Contaminated Aquatic Systems
421(4)
Questions and Problems
422(1)
References and Recommended Readings
423(2)
Beneficial and Pathogenic Microbes in Agriculture
Ian L. Pepper
Overview
425(4)
The Soil-Plant-Microorganism System
425(1)
The Rhizosphere Environment
425(1)
Organic Compounds Released by Plants
426(3)
Rhizosphere Populations
429(1)
Beneficial Root-Microbial Interactions
429(10)
Biological Dinitrogen Fixation
430(2)
Free-Living Dinitrogen Fixation
432(1)
Associative Dinitrogen Fixation
432(1)
The Legume-Rhizobia Symbioses
433(5)
Mycorrhizal Associations
438(1)
Pathogenic Microbes in Agriculture
439(8)
Plant Disease Caused by Fungi
440(1)
Diseases Caused by Bacteria
440(2)
Plant Diseases Caused by Viruses
442(1)
Soil Biological Control of Plant Diseases
443(2)
Questions and Problems
445(1)
References and Recommended Readings
445(2)
Environmentally Transmitted Pathogens
Patricia Rusin
Carlos E. Enriquez
Dana Johnson
Charles P. Gerba
Environmental Transmitted Pathogens
447(2)
Bacteria
449(10)
Salmonella
449(1)
Escherichia coli and Shigella
450(1)
Campylobacter
451(1)
Yersinia
452(1)
Vibrio
452(1)
Helicobacter
453(1)
Legionella
453(2)
Opportunistic Bacterial Pathogens
455(2)
Blue-Green Algae
457(2)
Parasitology
459(13)
Protozoa
460(4)
Nematodes
464(2)
Cestodes (Taenia saginata)
466(1)
Trematodes (Schistosoma mansoni)
467(2)
Emerging Pathogens
469(3)
Viruses
472(19)
Enteric Viruses
472(9)
Respiratory Viruses
481(3)
Questions and Problems
484(1)
References and Recommended Readings
485(6)
Indicator Microorganisms
Charles P. Gerba
The Concept of Indicator Organisms
491(1)
Total Coliforms
491(5)
The Most Probable Number (MPN) Test
493(1)
The Membrane Filter (MF) Test
493(1)
The Presence-Absence (P-A) Test
493(3)
Fecal Coliforms
496(1)
Fecal Streptococci
496(1)
Clostridium perfringens
497(1)
Heterotrophic Plate Count
497(2)
Bacteriophage
499(1)
Other Indicator Organisms
500(1)
Standards and Criteria for Indicators
500(5)
Questions and Problems
502(1)
References and Recommended Readings
503(2)
Domestic Wastes and Waste Treatment
Charles P. Gerba
Domestic Wastewater
505(3)
Modern Wastewater Treatment
508(12)
Primary Treatment
508(1)
Secondary Treatment
508(4)
Tertiary Treatment
512(1)
Removal of Pathogens by Sewage Treatment Processes
512(3)
Sludge Processing
515(1)
Pathogen Occurrence and Fate in Biosolids
516(2)
An Example of a Modern Sewage Treatment Plant
518(2)
Oxidation Ponds
520(1)
Septic Tanks
521(1)
Land Application of Wastewater
522(2)
Wetlands and Aquaculture Systems
524(4)
Solid Waste
528(7)
Municipal Solid Waste
528(1)
Modern Sanitary Landfills
528(2)
Composting of Biosolids and Domestic Solid Waste
530(2)
Questions and Problems
532(1)
References and Recommended Readings
533(2)
Drinking Water Treatment and Distribution
Charles P. Gerba
Water Treatment Processes
535(3)
Water Distribution Systems
538(1)
Assimilable Organic Carbon
539(4)
Questions and Problems
541(1)
References and Recommended Readings
541(2)
Disinfection
Charles P. Gerba
Thermal Destruction
543(1)
Kinetics of Disinfection
544(2)
Factors Affecting Disinfectants
546(1)
Halogens
547(3)
Chlorine
547(1)
Chloramines
548(1)
Chlorine Dioxide
549(1)
Bromine and Iodine
550(1)
Ozone
550(1)
Metal Ions
551(1)
Ultraviolet Disinfection
552(2)
Photodynamic Inactivation
554(1)
Gamma and High-Energy Irradiation
554(3)
Questions and Problems
555(1)
References and Recommended Readings
555(2)
Risk Assessment
Charles P. Gerba
The Concept of Risk Assessment
557(1)
Elements of Risk Assessment
557(3)
The Process of Risk Assessment
560(4)
Hazard Identification
560(1)
Exposure Assessment
560(2)
Dose-Response Assessment
562(1)
Risk Characterization
563(1)
Microbial Risk Assessment
564(6)
Questions and Problems
570(1)
References and Recommended Readings
570

An electronic version of this book is available through VitalSource.

This book is viewable on PC, Mac, iPhone, iPad, iPod Touch, and most smartphones.

By purchasing, you will be able to view this book online, as well as download it, for the chosen number of days.

Digital License

You are licensing a digital product for a set duration. Durations are set forth in the product description, with "Lifetime" typically meaning five (5) years of online access and permanent download to a supported device. All licenses are non-transferable.

More details can be found here.

A downloadable version of this book is available through the eCampus Reader or compatible Adobe readers.

Applications are available on iOS, Android, PC, Mac, and Windows Mobile platforms.

Please view the compatibility matrix prior to purchase.