Gene Probes 1 & 2 A Practical Approach 2-Volume Set
by Hames, B. D.; Higgins, S. J.Format: Hardcover
Pub. Date: 1996-02-15
Publisher(s): Oxford University Press
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Summary
Volume 1 (Preparation)
Introduction
1. Strategies for using gene probes
2. Preparation of nucleic acids for use as probes
3. DNA radiolabelling and detection
4. Non-radioactive labelling of
Table of Contents
| (Preparation) | |
| Introduction | |
| Strategies for using gene probes | |
| Preparation of nucleic acids for use as probes | |
| DNA radiolabelling and detection | |
| Non-radioactive labelling of DNA probes | |
| Preparation of synthetic oligodeoxynucleotide probes | |
| Probe preparation of PCR | |
| Synthesis of labelled riboprobes | |
| Antibody probes | |
| (Applications) | |
| Hyrbidization strategy | |
| Screening recombinant DNA libraries by hybriization and amplification | |
| Screening human recombinant libraries with nucleic acid probes | |
| Antibody screening of bacterial expression systems | |
| Isolation of cDNA clones by direct expression in mammalian cells | |
| Genomic mapping and gene structure analysis | |
| Analysis of gene expression by blotting techniques | |
| In situ hybridization of nucleic acid probes to cellular RNA | |
| Applications of chromosomal in situ hybridization | |
| Analysis of gene expression analysis by PCR: a quantitative approach | |
| Analysis of protein expression by immunocytochemistry | |
| Gene targeting in mammalian cells | |
| Directed mutageneis and mutant analysis | |
| Human gene mutational analysis | |
| Table of Contents provided by Publisher. All Rights Reserved. |
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