| Editorial Board |
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xiii | (2) |
| Contributors |
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xv | (10) |
| Preface |
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xxv | |
| PART I GENERAL METHODS |
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1 | (444) |
| SECTION A IMMUNOLOGICAL METHODS |
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3 | (72) |
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5 | (2) |
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2 Agglutination Immunoassays |
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7 | (6) |
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3 Light-Scattering Immunoassays |
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13 | (7) |
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4 Enzyme-Linked Immunoassays |
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20 | (10) |
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5 A Model System for Radioimmunoassays: Digoxin Reference Method |
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30 | (9) |
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6 Fluorescence Immunoassays |
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39 | (10) |
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7 Chemiluminescence Immunoassays |
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49 | (5) |
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8 Immunofixation and Immunoblotting |
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54 | (11) |
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9 Clinical Indications for Electrophoresis and Immunofixation |
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65 | (10) |
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| SECTION B MOLECULAR BIOLOGY METHODS |
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75 | (56) |
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10 Introduction to Molecular Methodology |
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77 | (31) |
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11 Choosing Appropriate Methodologies in the Clinical Environment: Immunological Techniques versus Molecular Methods |
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108 | (8) |
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12 Keys to Successful In Situ PCR |
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116 | (15) |
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| SECTION C IMMUNOGLOBULIN METHODS |
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131 | (46) |
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133 | (1) |
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14 Immunoglobulin Quantitation |
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134 | (13) |
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147 | (9) |
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16 Immunochemical Characterization of Immunoglobulins |
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156 | (21) |
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| SECTION D COMPLEMENT, IMMUNE COMPLEXES, AND CRYOGLOBULIN |
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177 | (48) |
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179 | (2) |
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181 | (6) |
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187 | (5) |
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192 | (11) |
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21 Genetic Typing of the Complement System |
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203 | (5) |
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22 Tests for Circulating Immune Complexes and Autoantibodies to C1q |
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208 | (9) |
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217 | (8) |
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| SECTION E IMMUNE CELL PHENOTYPING AND FLOW CYTOMETRIC ANALYSIS |
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225 | (48) |
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227 | (2) |
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25 Immunophenotyping by Flow Cytometry |
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229 | (16) |
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26 Functional Assays by Flow Cytometry |
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245 | (10) |
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27 Flow Cytometric Analysis of DNA Content in Solid Tumors |
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255 | (9) |
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28 Emerging Clinical Applications of Flow Cytometry: Microbiology and Parasitology |
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264 | (9) |
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| SECTION F IMMUNE CELL ASSAYS |
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273 | (72) |
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275 | (1) |
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30 Delayed Hypersensitivity Skin Testing |
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276 | (5) |
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31 Cryopreservation of Peripheral Blood Mononuclear Cells |
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281 | (6) |
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32 T-Lymphocyte Activation |
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287 | (9) |
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33 Cytolytic Cell Functions |
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296 | (8) |
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34 Methods for Identification of the T-Cell Antigen Receptor |
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304 | (9) |
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35 Lymphocyte Proliferation |
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313 | (7) |
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36 Phagocytic Cell Functions. I. Oxidation and Chemotaxis |
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320 | (9) |
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37 Phagocytic Cell Functions. II. Adhesion and Phagocytosis |
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329 | (5) |
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38 Methods for Analysis of Apoptosis by Flow Cytometry |
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334 | (11) |
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| SECTION G IMMUNE CELL PRODUCTS |
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345 | (20) |
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347 | (1) |
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40 Cytokines and Immune Cell Products |
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348 | (9) |
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41 Development and Use of Cytokine and Growth Factor Potency Standards |
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357 | (3) |
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42 Measuring Cell Adhesion Molecules |
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360 | (5) |
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| SECTION H IMMUNOHISTOLOGY AND IMMUNOPATHOLOGY |
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365 | (80) |
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367 | (2) |
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44 Immunohistochemistry: Principles and Practice |
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369 | (11) |
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45 Immunohistochemistry in the Diagnosis of Solid Tumors |
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380 | (9) |
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46 Immunohistochemistry in the Diagnosis of Lymphomas |
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389 | (11) |
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47 Prognostic Markers in Immunohistochemistry |
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400 | (14) |
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48 In Situ Hybridization as an Adjunct Method with Immunohistochemistry |
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414 | (8) |
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49 Immunofluorescence Methods for Diagnosis of Renal and Skin Diseases |
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422 | (9) |
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50 Immunoelectron Microscopy in Diagnostic Pathology |
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431 | (14) |
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| PART II IMMUNOLOGIC DIAGNOSIS AND MONITORING OF DISEASE |
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445 | (708) |
| SECTION I INFECTIOUS DISEASES CAUSED BY BACTERIA, MYCOPLASMAS, CHLAMYDIAE, AND RICKETTSIAE |
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447 | (124) |
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449 | (1) |
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52 Immune Response to Streptococcal Antigens: Diagnostic Methods |
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450 | (8) |
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53 Haemophilus influenzae Type b, Neisseria meningitidis, Streptococcus pneumoniae, and Corynebacterium diphtheriae Vaccines |
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458 | (12) |
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54 Clostridium tetani: Immune Response and Diagnostic Methods |
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470 | (3) |
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55 Immunologic Methods for Diagnosis of Infections by Gram-Negative Cocci |
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473 | (11) |
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56 Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic Members of the Families Enterobacteriaceae and Vibrionaceae |
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484 | (14) |
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57 Serodiagnosis of Helicobacter pylori Infection |
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498 | (4) |
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58 Detection of Antibodies to Legionella spp. |
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502 | (8) |
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59 Immunologic Methods for Diagnosis of Spirochetal Diseases |
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510 | (16) |
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60 Lyme Disease: Serodiagnosis of Borrelia burgdorferi Sensu Lato Infection |
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526 | (8) |
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61 Immunological Procedures for Diagnosis of Tuberculosis and Leprosy |
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534 | (9) |
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62 Nocardiae and Agents of Actinomycosis |
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543 | (4) |
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63 Mycoplasma Infection: Immunologic and Molecular Biologic Diagnostic Techniques |
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547 | (5) |
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552 | (6) |
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65 Immunoserology of Rickettsiae |
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558 | (13) |
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| SECTION J MYCOTIC AND PARASITIC DISEASES |
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571 | (34) |
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66 Introduction to Clinical Immunomycology and Clinical Immunoparasitology |
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573 | (2) |
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67 Clinical Immunoparasitology |
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575 | (10) |
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68 Clinical Immunomycology |
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585 | (20) |
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| SECTION K VIRAL DISEASES |
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605 | (156) |
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607 | (1) |
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608 | (10) |
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71 Antibody-Enhanced Detection of Viruses in Cell Cultures |
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618 | (7) |
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625 | (6) |
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73 Varicella-Zoster Virus |
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631 | (3) |
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634 | (10) |
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644 | (5) |
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76 Human Herpesvirus 6 and 7 |
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649 | (6) |
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655 | (6) |
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661 | (6) |
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667 | (6) |
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673 | (6) |
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81 Respiratory Syncytial Virus and the Parainfluenza Viruses |
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679 | (9) |
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688 | (5) |
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693 | (6) |
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699 | (3) |
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702 | (17) |
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86 Enzyme Immunoassays for Detection of Rotavirus Antigen and Antibody |
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719 | (10) |
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729 | (8) |
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737 | (5) |
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89 Marburg Virus, Ebola Virus, and the Arenaviruses |
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742 | (13) |
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755 | (6) |
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| SECTION L HUMAN IMMUNODEFICIENCY VIRUS |
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761 | (50) |
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763 | (1) |
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92 Immunologic Evaluation of Patients with Human Immunodeficiency Virus Infection |
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764 | (9) |
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93 Quantitation of Viremia in Human Immunodeficiency Virus Infection by Viral RNA Estimation |
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773 | (8) |
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94 Isolation and Detection of Human Immunodeficiency Virus |
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781 | (7) |
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95 Principles and Procedures of Human Immunodeficiency Virus Serodiagnosis |
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788 | (14) |
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96 Sequence Analysis of Human Immunodeficiency Virus Type 1 Haplotypes |
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802 | (9) |
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| SECTION M IMMUNODEFICIENCY DISEASES |
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811 | (54) |
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CHARLOTTE CUNNINGHAMRUNDLES |
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813 | (2) |
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CHARLOTTE CUNNINGHAM-RUNDLES |
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98 Genetics of Inherited Immunodeficiency Diseases and Diagnostic Techniques |
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815 | (9) |
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99 Combined B- and T-Cell Deficiencies |
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824 | (5) |
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100 Investigation of Signal Transduction Defects |
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829 | (6) |
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101 Primary Antibody Deficiencies in Children and Adults |
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835 | (6) |
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102 Inherited and Acquired C1-Inhibitor Deficiency |
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841 | (4) |
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103 Genetically Determined Deficiencies of the Complement System: C1, C4, C2, and C3 |
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845 | (5) |
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104 Alternative Pathway and Late Complement Component Deficiencies |
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850 | (5) |
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105 Neutropenia and Neutrophil Defects |
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855 | (10) |
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| SECTION N ALLERGIC DISEASES |
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865 | (52) |
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867 | (1) |
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107 Quantitation and Standardization of Allergens |
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868 | (7) |
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108 In Vivo Diagnostic Allergy Testing Methods |
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875 | (6) |
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109 Immunological Tests for Diagnosis and Management of Human Allergic Disease: Total and Allergen-Specific IgE and Allergen-Specific IgG |
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881 | (12) |
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N. FRANKLIN ADKINSON, JR. |
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110 Tests for Immunological Reactions to Drugs and Occupational Allergens |
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893 | (6) |
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N. FRANKLIN ADKINSON, JR. |
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111 Assay Methods for Measurement of Mediators and Markers of Allergic Inflammation |
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899 | (9) |
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112 Immunological Tests for Evaluation of Hypersensitivity Pneumonitis and Allergic Bronchopulmonary Aspergillosis |
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908 | (9) |
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| SECTION O SYSTEMIC AUTOIMMUNE DISEASES |
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917 | (52) |
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919 | (1) |
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114 Immunofluorescent Antinuclear Antibody Tests |
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920 | (8) |
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115 Detection of Autoantibodies to Ribonucleoprotein Particles by Immunoblotting |
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928 | (7) |
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116 Enzyme-Linked Immunosorbent Assays for Antibodies to Native DNA, Histones, and (H2A-H2B)-DNA |
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935 | (7) |
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942 | (7) |
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118 Antiphospholipid Antibodies |
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949 | (5) |
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119 Antineutrophil Cytoplasmic Antibodies |
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954 | (6) |
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120 Autoantibodies to Complement Components |
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960 | (9) |
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| SECTION P ORGAN-LOCALIZED AUTOIMMUNE DISEASES |
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969 | (62) |
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971 | (1) |
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972 | (11) |
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123 Detection of Autoantibodies in Myasthenia Gravis and Lambert-Eaton Myasthenic Syndrome |
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983 | (6) |
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124 Detection of Antimitochondrial Autoantibodies in Primary Biliary Cirrhosis and Liver-Kidney Microsomal Antibodies in Autoimmune Hepatitis |
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989 | (8) |
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997 | (8) |
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126 Cardiovascular Diseases |
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1005 | (3) |
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127 Kidney and Lung Disease Mediated by Glomerular Basement Membrane Antibodies: Detection by Western Blot Analysis |
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1008 | (5) |
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128 Human Spermatozoa Antibodies: Detection and Clinical Significance |
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1013 | (5) |
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129 Autoimmune Hemolytic Anemias |
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1018 | (8) |
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130 Autoimmune Thrombocytopenia |
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1026 | (5) |
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| SECTION Q CANCER |
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1031 | (44) |
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1033 | (2) |
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132 Human Tumor Antigens Recognized by T Lymphocytes |
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1035 | (6) |
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1041 | (7) |
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134 Cancer Genes and Early Cancer Detection |
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1048 | (7) |
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135 Malignancies of the Lymphoid and Histiocytic Systems |
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1055 | (10) |
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136 Monitoring of Immunologic Therapies |
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1065 | (10) |
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| SECTION R TRANSPLANTATION IMMUNOLOGY AND IMMUNOGENETICS |
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1075 | (78) |
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137 Introduction: Genes in the Human Major Histocompatibility Complex Relevant to Immune Regulation and Transplantation and Other Clinical Applications |
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1077 | (3) |
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138 Histocompatibility Testing by Immunologic Methods: Cellular Assays |
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1080 | (7) |
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139 Histocompatability Testing by Immunologic Methods: Humoral Assays |
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1087 | (11) |
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140 HLA Typing by Molecular Methods |
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1098 | (14) |
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141 Guidelines for Use of Immunogenetic Tests in Organ Transplantation |
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1112 | (5) |
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142 Immunological Characterization of Solid Organ Graft Recipients |
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1117 | (6) |
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143 Histocompatibility Assessment in Bone Marrow Transplantation |
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1123 | (9) |
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144 Statistical Analysis and Applications of HLA Population Data |
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1132 | (9) |
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145 Principles and Applications of Genetic Identification |
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1141 | (12) |
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| PART III ADMINISTRATION |
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1153 | (70) |
| SECTION S LABORATORY MANAGEMENT |
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1155 | (68) |
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1157 | (1) |
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147 Establishment and Verification of Reference Values |
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1158 | (10) |
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148 Standardization of Immunoassay Methodologies |
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1168 | (12) |
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1180 | (11) |
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150 Planning Statistical Quality Control Procedures |
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1191 | (10) |
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151 Interlaboratory Proficiency Testing in Laboratory Immunology |
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1201 | (5) |
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152 Clinical Significance and Cost-Effectiveness of Clinical Immunology Testing |
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1206 | (7) |
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1213 | (10) |
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| Author Index |
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1223 | (2) |
| Subject Index |
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1225 | |
Other versions by this Author
